Clue is a full-motion video adaptation of the popular board game of the same name. It is an amalgamation of two earlier CD-i titles where (up to six) players take turns investigating a mansion where a murder happened, uncovering clues and evidence to reveal the assassin. There are three storylines with four difficulty levels, each with a unique solution making it altogether twelve different mysteries: Deadly Patent (from Clue), The Road to Damascus and Blackmail (both from Clue 2: The Mysteries Continue).

The gameplay remains identical to the original games and stays near the board game: During each turn, the player can do two moves: moving between rooms, interrogating a suspect, watching a flashback video right before the murder (depends on the room) or examining an item. The goal is to be able to name the murderer, the crime scene and the murder weapon.