Based on the award-winning TV series, The Adventures of Batman and Robin pits the player against the diabolical Mr. Freeze who wants to freeze Gotham with a huge ice cannon and his hired help of other Gotham super villains: The Joker, Mad Hatter, and Two-Face. The player can take the role of either Batman or Robin but this choice doesn't make a difference regarding gameplay.

This is a side-scrolling action game. The player basically walks from left to right while fighting numerous foes in hand-to-hand combat. Weapons which are found during the levels are used to shoot enemies from a greater distance. These weapons can also be upgraded by picking up the corresponding symbols.

There are overall four areas (one for each villain) with three levels each, including a film studio or the Gotham University. While most stages are typical fighting levels with occasional 3D effects there are also a few flying ones where the player uses the Batwing or a jet pack.